Aberrant mitosis, light microscopy
Fluorescent light microscopy of a cultured mammalian cancer cell undergoing mitosis (nuclear division) and cytokinesis (cell division). Mitosis is the formation of two daughter nuclei from one parent nucleus. Fluorescent markers have been used to highlight DNA (deoxyribonucleic acid, pink) and alpha tubulin (green), a component of microtubules. The cycle is first seen in interphase with its nucleus clearly visible. At prophase, the nuclear envelope dissolves and the chromosomes condense. The cell progress through prometaphase to metaphase, where the chromosomes align along the centre of the cell. During anaphase the chromosomes start to move to the two opposite poles, guided by microtubules. However, in this cell, there are three poles, which will lead to missegregation. The last stage of mitosis is telophase, when the separated chromosomes have moved to different ends of the cell and three new nuclei (instead of the usual two) form around them. Cytokinesis divides the cytoplasm of the cells, separating them. Filmed over 2 hours.